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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PÉREZ, C.A.; WINGFIELD, M.J.; SLIPPERS, B.; ALTIER, N.; BLANCHETTE, R.A. |
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Afiliación : |
C.A. PÉREZ, Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA; Departamento de Protección Vegetal, EEMAC, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; M.J. WINGFIELD, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa; B. SLIPPERS, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa; NORA ADRIANA ALTIER MANZINI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; R.A. BLANCHETTE, Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA. |
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Título : |
Endophytic and canker-associated Botryosphaeriaceae occurring on non-native Eucalyptus and native Myrtaceae trees in Uruguay. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
Fungal Diversity, 2010, v. 41 no.1, p. 53-69. |
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ISSN : |
1560-2745 |
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DOI : |
10.1007/s13225-009-0014-8 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 August 2009 / Accepted: 7 October 2009 / Published online: 15 January 2010. |
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Contenido : |
ABSTRACT.
Species of the Botryosphaeriaceae are important pathogens causing cankers and die-back on many woody plants. In Uruguay, Neofusicoccum eucalyptorum, N. ribis and B. dothidea have previously been associated with stem cankers on plantation-grown Eucalyptus globulus. However, very little is known regarding the occurrence and species diversity of Botryosphaeriaceae in native Myrtaceae forests or what their relationship is to those species infecting Eucalyptus in plantations. The objectives of this study were to identify the Botryosphaeriaceae species present as endophytes or associated with cankers in both introduced and native tree hosts in Uruguay, and to test the pathogenicity of selected isolates obtained from native trees on Eucalyptus. Symptomatic and asymptomatic material was collected countrywide from Eucalyptus plantations and native Myrtaceae trees. Single spore cultures were identified based on conidial morphology and comparisons of DNA sequences of the ITS and EF1-α regions. Six Botryosphaeriaceae species were identified. Botryosphaeria dothidea, N. eucalyptorum and specimens residing in the N. parvum-N. ribis complex were isolated from both introduced Eucalyptus and native Myrtaceae trees, whereas Lasiodiplodia pseudotheobromae was found only on Myrcianthes pungens. Diplodia pseudoseriata sp. nov. and Spencermartinsia uruguayensis sp. nov. are novel species found only on native myrtaceous hosts. Pathogenicity tests showed that isolates obtained from native trees and identified as L. pseudotheobromae, N. eucalyptorum and the N. parvum-N.ribis complex are pathogenic to E. grandis. Interestingly, Lasiodiplodia pseudotheobromae has not previously been found on Eucalyptus in Uruguay and represents a potential threat to this host.
© Kevin D. Hyde 2010. MenosABSTRACT.
Species of the Botryosphaeriaceae are important pathogens causing cankers and die-back on many woody plants. In Uruguay, Neofusicoccum eucalyptorum, N. ribis and B. dothidea have previously been associated with stem cankers on plantation-grown Eucalyptus globulus. However, very little is known regarding the occurrence and species diversity of Botryosphaeriaceae in native Myrtaceae forests or what their relationship is to those species infecting Eucalyptus in plantations. The objectives of this study were to identify the Botryosphaeriaceae species present as endophytes or associated with cankers in both introduced and native tree hosts in Uruguay, and to test the pathogenicity of selected isolates obtained from native trees on Eucalyptus. Symptomatic and asymptomatic material was collected countrywide from Eucalyptus plantations and native Myrtaceae trees. Single spore cultures were identified based on conidial morphology and comparisons of DNA sequences of the ITS and EF1-α regions. Six Botryosphaeriaceae species were identified. Botryosphaeria dothidea, N. eucalyptorum and specimens residing in the N. parvum-N. ribis complex were isolated from both introduced Eucalyptus and native Myrtaceae trees, whereas Lasiodiplodia pseudotheobromae was found only on Myrcianthes pungens. Diplodia pseudoseriata sp. nov. and Spencermartinsia uruguayensis sp. nov. are novel species found only on native myrtaceous hosts. Pathogenicity tests showed that isolates obtained from ... Presentar Todo |
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Thesagro : |
AGENTES PATÓGENOS; BOTRYOSPHAERIA; ENFERMEDADES FORESTALES; PATÓGENOS FORESTALES. |
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Asunto categoría : |
K10 Producción forestal |
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Marc : |
LEADER 02678naa a2200253 a 4500
001 1051308
005 2019-10-15
008 2010 bl uuuu u00u1 u #d
022 $a1560-2745
024 7 $a10.1007/s13225-009-0014-8$2DOI
100 1 $aPÉREZ, C.A.
245 $aEndophytic and canker-associated Botryosphaeriaceae occurring on non-native Eucalyptus and native Myrtaceae trees in Uruguay.$h[electronic resource]
260 $c2010
500 $aArticle history: Received: 19 August 2009 / Accepted: 7 October 2009 / Published online: 15 January 2010.
520 $aABSTRACT. Species of the Botryosphaeriaceae are important pathogens causing cankers and die-back on many woody plants. In Uruguay, Neofusicoccum eucalyptorum, N. ribis and B. dothidea have previously been associated with stem cankers on plantation-grown Eucalyptus globulus. However, very little is known regarding the occurrence and species diversity of Botryosphaeriaceae in native Myrtaceae forests or what their relationship is to those species infecting Eucalyptus in plantations. The objectives of this study were to identify the Botryosphaeriaceae species present as endophytes or associated with cankers in both introduced and native tree hosts in Uruguay, and to test the pathogenicity of selected isolates obtained from native trees on Eucalyptus. Symptomatic and asymptomatic material was collected countrywide from Eucalyptus plantations and native Myrtaceae trees. Single spore cultures were identified based on conidial morphology and comparisons of DNA sequences of the ITS and EF1-α regions. Six Botryosphaeriaceae species were identified. Botryosphaeria dothidea, N. eucalyptorum and specimens residing in the N. parvum-N. ribis complex were isolated from both introduced Eucalyptus and native Myrtaceae trees, whereas Lasiodiplodia pseudotheobromae was found only on Myrcianthes pungens. Diplodia pseudoseriata sp. nov. and Spencermartinsia uruguayensis sp. nov. are novel species found only on native myrtaceous hosts. Pathogenicity tests showed that isolates obtained from native trees and identified as L. pseudotheobromae, N. eucalyptorum and the N. parvum-N.ribis complex are pathogenic to E. grandis. Interestingly, Lasiodiplodia pseudotheobromae has not previously been found on Eucalyptus in Uruguay and represents a potential threat to this host. © Kevin D. Hyde 2010.
650 $aAGENTES PATÓGENOS
650 $aBOTRYOSPHAERIA
650 $aENFERMEDADES FORESTALES
650 $aPATÓGENOS FORESTALES
700 1 $aWINGFIELD, M.J.
700 1 $aSLIPPERS, B.
700 1 $aALTIER, N.
700 1 $aBLANCHETTE, R.A.
773 $tFungal Diversity, 2010$gv. 41 no.1, p. 53-69.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
14/09/2017 |
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Actualizado : |
13/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - A |
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Autor : |
VIKRAM, A.; ROVIRA, P.J.; AGGA, G.E.; ARTHUR, T.M.; BOSILEVAC, J.M.; WHEELER, T.L.; MORLEY, P.S.; BELK, K.E.; SCHMIDT, J.W. |
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Afiliación : |
AMIT VIKRAM, U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, NE.; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Department of Animal Sciences Colorado State University, CO, US.; GETHYM E. AGGA, U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, NE.; TERRANCE M. ARTHUR, U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, NE.; JOSEPH M. BOSILEVAC, U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, NE.; TOMMY L. WHEELER, U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, NE.; PAUL S. MORLEY, Department of Animal Sciences Colorado State University, CO, US. Department of Clinical Sciences, College of Veterinary Medicine, Colorado State University, CO, US.; KEITH E. BELK, Department of Animal Sciences Colorado State University, CO, US.; JOHN W. SCHMIDT, U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, NE. Department of Animal Sciences Colorado State University, CO, US. |
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Título : |
Impact of "Raised Without Antibiotics" beef cattle production practices on occurrences of antimicrobial resistance. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Applied and Environmental Microbiology, 2017, v. 83, no. 22, e01682-17. |
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DOI : |
10.1128/AEM.01682-17 |
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Idioma : |
Inglés |
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Notas : |
Article history: Accepted manuscript posted online 8 September 2017// published in november 2017. |
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Contenido : |
The specific antimicrobial resistance (AMR) decreases that can be expected from reducing antimicrobial (AM) use in United States (US) beef production have not been defined. To address this data gap, feces were recovered from 36 lots of ?raised without antibiotics? (RWA) and 36 lots of ?conventional? (CONV) beef cattle. Samples (N = 719) were collected during harvest and distributed over a year. AMR was assessed by: i) culture of six AM-resistant bacteria (ARB); ii) qPCR for 10 AMR genes (ARGs); iii) an qPCR array of 84 ARGs; and iv) metagenomic sequencing. Generally, AMR levels were similar, but some were higher in CONV. The prevalence of third-generation cephalosporin-resistant (3GCr) Escherichia coli was marginally different between production systems (47.5 % CONV, 34.8% RWA, P = 0.04), but the seasonal effect (92.8% summer, 48.3% winter, P < 0.01) was greater. Erythromycin-resistant (ERYr) Enterococcus spp. concentrations significantly differed between production systems (CONV = 1.91 log10 CFU/g, RWA = 0.73 log10 CFU/g, P < 0.01). Levels of aadA1, ant(6)-I, blaACI, erm(A), erm(B), erm(C), erm(F), erm(Q), tet(A), tet(B), tet(M), and tet(X) ARGs were higher (P < 0.05) in CONV. Aggregate abundances of all 43 ARGs detected by metagenomic sequencing and the aggregate abundances of ARGs in the aminoglycoside, ?-lactam, macrolide-lincosamide-streptogramin B (MLSB), and tetracycline AM classes did not differ (log2 fold change < 1.0) between CONV and RWA. These results suggest that further reductions of AM use in US beef cattle production may not yield significant AMR reductions beyond MLSB and tetracycline resistance. MenosThe specific antimicrobial resistance (AMR) decreases that can be expected from reducing antimicrobial (AM) use in United States (US) beef production have not been defined. To address this data gap, feces were recovered from 36 lots of ?raised without antibiotics? (RWA) and 36 lots of ?conventional? (CONV) beef cattle. Samples (N = 719) were collected during harvest and distributed over a year. AMR was assessed by: i) culture of six AM-resistant bacteria (ARB); ii) qPCR for 10 AMR genes (ARGs); iii) an qPCR array of 84 ARGs; and iv) metagenomic sequencing. Generally, AMR levels were similar, but some were higher in CONV. The prevalence of third-generation cephalosporin-resistant (3GCr) Escherichia coli was marginally different between production systems (47.5 % CONV, 34.8% RWA, P = 0.04), but the seasonal effect (92.8% summer, 48.3% winter, P < 0.01) was greater. Erythromycin-resistant (ERYr) Enterococcus spp. concentrations significantly differed between production systems (CONV = 1.91 log10 CFU/g, RWA = 0.73 log10 CFU/g, P < 0.01). Levels of aadA1, ant(6)-I, blaACI, erm(A), erm(B), erm(C), erm(F), erm(Q), tet(A), tet(B), tet(M), and tet(X) ARGs were higher (P < 0.05) in CONV. Aggregate abundances of all 43 ARGs detected by metagenomic sequencing and the aggregate abundances of ARGs in the aminoglycoside, ?-lactam, macrolide-lincosamide-streptogramin B (MLSB), and tetracycline AM classes did not differ (log2 fold change < 1.0) between CONV and RWA. These results suggest tha... Presentar Todo |
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Palabras claves : |
ANTIMICROBIAL RESISTANCE; BACTERIAL CULTURE; BEEF CATTLE PRODUCTION; METAGENOMICS; RAISED WITHOUT ANTIBIOTICS. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02646naa a2200301 a 4500
001 1057565
005 2018-09-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1128/AEM.01682-17$2DOI
100 1 $aVIKRAM, A.
245 $aImpact of "Raised Without Antibiotics" beef cattle production practices on occurrences of antimicrobial resistance.$h[electronic resource]
260 $c2017
500 $aArticle history: Accepted manuscript posted online 8 September 2017// published in november 2017.
520 $aThe specific antimicrobial resistance (AMR) decreases that can be expected from reducing antimicrobial (AM) use in United States (US) beef production have not been defined. To address this data gap, feces were recovered from 36 lots of ?raised without antibiotics? (RWA) and 36 lots of ?conventional? (CONV) beef cattle. Samples (N = 719) were collected during harvest and distributed over a year. AMR was assessed by: i) culture of six AM-resistant bacteria (ARB); ii) qPCR for 10 AMR genes (ARGs); iii) an qPCR array of 84 ARGs; and iv) metagenomic sequencing. Generally, AMR levels were similar, but some were higher in CONV. The prevalence of third-generation cephalosporin-resistant (3GCr) Escherichia coli was marginally different between production systems (47.5 % CONV, 34.8% RWA, P = 0.04), but the seasonal effect (92.8% summer, 48.3% winter, P < 0.01) was greater. Erythromycin-resistant (ERYr) Enterococcus spp. concentrations significantly differed between production systems (CONV = 1.91 log10 CFU/g, RWA = 0.73 log10 CFU/g, P < 0.01). Levels of aadA1, ant(6)-I, blaACI, erm(A), erm(B), erm(C), erm(F), erm(Q), tet(A), tet(B), tet(M), and tet(X) ARGs were higher (P < 0.05) in CONV. Aggregate abundances of all 43 ARGs detected by metagenomic sequencing and the aggregate abundances of ARGs in the aminoglycoside, ?-lactam, macrolide-lincosamide-streptogramin B (MLSB), and tetracycline AM classes did not differ (log2 fold change < 1.0) between CONV and RWA. These results suggest that further reductions of AM use in US beef cattle production may not yield significant AMR reductions beyond MLSB and tetracycline resistance.
653 $aANTIMICROBIAL RESISTANCE
653 $aBACTERIAL CULTURE
653 $aBEEF CATTLE PRODUCTION
653 $aMETAGENOMICS
653 $aRAISED WITHOUT ANTIBIOTICS
700 1 $aROVIRA, P.J.
700 1 $aAGGA, G.E.
700 1 $aARTHUR, T.M.
700 1 $aBOSILEVAC, J.M.
700 1 $aWHEELER, T.L.
700 1 $aMORLEY, P.S.
700 1 $aBELK, K.E.
700 1 $aSCHMIDT, J.W.
773 $tApplied and Environmental Microbiology, 2017$gv. 83, no. 22, e01682-17.
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